By Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung
This handbook presents technical protocols for musculoskeletal study on a translational foundation, i.e. a disease-orientated strategy. It deals assistance on a number of laboratory innovations, together with cellphone tradition and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal types, CT- and MRI-based densitometry and microarchitectural research, biomechanics and sensible research of orthopedic kinesiology, and so forth. The content material is straightforward and simple, with illustrations and step by step systems as a simple experimental reference for team of workers in easy and scientific musculoskeletal examine and schooling. This publication will offer a distinct multidisciplinary platform for numerous professions -- not just orthopedics, but additionally biomedical engineering and biomaterial sciences -- related to either easy and scientific medication.
Contents: phone tradition and Molecular Biology; Histology and Histomorphometry; Microscopy and Bioimaging; Laboratory Animal types; X-Ray- and MRI-based Densitometry; Biomechanics and movement research
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Extra resources for A Practical Manual for Musculoskeletal Research
The software for initial image processing is normally provided with the scanner; this allows the boundaries of individual spots to be determined and the total signal intensity to be measured over the whole spot (signal volume). The signal intensity should be corrected for background, and control measures should be included to measure nonspecific hybridization and variable hybridization across arrays. The aim of data mining is to convert the hybridization signals into numbers which can be used to build a gene expression matrix.
Failure to remove the cover slip immediately may lead to elevated background fluorescence. Cover slips must be free of oils, dust, and other contaminants. Lower the cover slip onto the microarray from left to right. Once it touches the liquid on the array, release it quickly so that the sample pushes out air bubbles as it forms a monolayer against the microarray surface. Small air bubbles trapped under the cover slip exit after several minutes at 62°C. h The microarray should be transferred quickly from the cassette to the washing buffer.
At the very least, one collaborative research group should have one expert with the needed background, experience, and understanding. qxd 8/12/2008 12:06 PM Page 9 DNA Microarray 9 Fig. 2. Steps in the implementation of a DNA microarray experiment. Cy3 and Cy5 are the cyanine dyes (green and red, respectively) used to label the cDNAs. 1. g. mammalian cultured cells grown in a monolayer): • b Rinse the cell monolayer with ice-cold phosphate buffered saline (PBS) three times. There are several well-validated commercial kits for extracting intact mRNA populations from cells in such a way as to give us consistent reliable preparations free from contaminating DNA and protein.