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By Ronald W. Estabrook and Paul Srere (Eds.)

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Metzenberg, R. , Hall, L. , and Cohen, P. , J . Biol. Chem. 229, 1019-1025 (1957). 25. ,Eur. J. Biochem. 7, 119-127 (1968). 26. , Biochem. J. 127, 503-508 (1972). 27. , and Jones, M. , Arch. Biochem. Biophys. 175, 270-278 (1976). 28. ,Eur. J. Biochem. 85, 373-383 (1978). 29. Elliott, K. R. , and Tipton, K. , FEBS Lett. 37, 7 9 - 8 1 (1973). 30. , and Cohen, P. , J. Biol. Chem. 235, 718-724 (1961). 30a. Strahler, J. D. Thesis, University of Wisconsin, Madison, 1974. 30b. Strahler, J. , Fed. Proc, Fed.

Wyngaarden, and S. S. , pp. 362-386. McGraw-Hill, New York, 1978. 116. Brusilow, S. , Valle, D. , and Batshaw, M. , Lancet 1, 452-454 (1979). 117. , Le Van, S. , Trend, B. , and Russell, L. , Proc. NatL Acad. Sei. A. 73, 1693-1697 (1976). DISCUSSION Dr. Lane: Do you know what the rate-limiting step is in the cycle? Dr. Cohen: Argininosuccinate synthetase activity is of the same order as that of carbamyl phosphate synthetase. However, because CPS I represents the initial step in urea biosynthesis, this step can be considered to be rate-limiting under normal conditions.

102. , and Cohen, P. ,Proc. NatL Acad. Sei. A. 76, 5071-5075 (1979). 103. Conboy, J. , Kalousek, R, and Rosenberg, L. ,Proc. NatL Acad. Sei. A. 76, 5724-5727 (1979). 104. , and Cohen, P. , J. Biochem. {Tokyo) 88, 18291836 (1980). 105. , and Cohen, P. ,Proc. NatL Acad. Sei. A. 77, 7044-7048(1980). 106. , and Cohen, P. , J . Biol. , 256,4127-4132 (1981). 107. Cohen, P. , in "The Urea Cycle" (S. Grisolia, R. Baguena, and F. ), pp. 21-38. Wiley, New York, 1976. 108. , Paik, W. , and Cohen, P. yProc.

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